quantitative sequenom massarray assay Search Results


massarray platform  (CapitalBio Corporation)
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CapitalBio Corporation massarray platform
Massarray Platform, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequenom massarray platform  (CapitalBio Corporation)
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CapitalBio Corporation sequenom massarray platform
Sequenom Massarray Platform, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray quantitative methylation analysis genomic dna  (Sequenom)
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Sequenom massarray quantitative methylation analysis genomic dna
Massarray Quantitative Methylation Analysis Genomic Dna, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bac acgh analysis sequenom massarray system qpcr  (Sequenom)
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Sequenom bac acgh analysis sequenom massarray system qpcr
Bac Acgh Analysis Sequenom Massarray System Qpcr, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray platform  (Sequenom)
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Sequenom massarray platform
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Massarray Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray epityper platform  (Sequenom)
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Sequenom massarray epityper platform
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Massarray Epityper Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray system  (Sequenom)
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Sequenom massarray system
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Massarray System, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epityper assay  (Sequenom)
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Sequenom epityper assay
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Epityper Assay, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qpcr results  (Sequenom)
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Sequenom qpcr results
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Qpcr Results, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primer assays  (TIB MOLBIOL)
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TIB MOLBIOL primer assays
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
Primer Assays, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray compact maldi tof  (Sequenom)
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Sequenom massarray compact maldi tof
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
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epityper software v1 0  (Sequenom)
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Sequenom epityper software v1 0
Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and <t>MassARRAY]</t> analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.
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Image Search Results


Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and MassARRAY] analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.

Journal: Cancer Research

Article Title: Xenoestrogen-Induced Epigenetic Repression of microRNA-9-3 in Breast Epithelial Cells

doi: 10.1158/0008-5472.can-08-4914

Figure Lengend Snippet: Figure 1. Preexposure of MDECs to diethylstilbestrol and immunofluorescence analysis of nuclear ER-a. A, treatment scheme. Breast progenitor cells were propagated as nonadherent spherical colonies, called mammospheres, and treated with 70 nmol/L diethylstilbestrol or DMSO solvent control for 3 wk. To induce differentiation, cells were seeded on a collagen substratum in the absence of diethylstilbestrol for 3 wk. Expression profiling (microRNA microarray), immunofluorescence image (IFA), and epigenetic [chromatin immunoprecipitation-PCR (ChIP-PCR) and MassARRAY] analyses were then done on the progeny epithelial cells. B, increased internalization of ER-a in diethylstilbestrol-preexposed MDECs. After the preexposure to diethylstilbestrol (DES) or DMSO, MDECs were subjected to immunofluorescence analysis. The percentage of subcellular localization of ER-a–positive cells, independently scored by two researchers, is shown in the bar chart. Columns, mean of five independent sets of MDEC samples; bars, SE. P < 0.001 (Student’s t test). C, nuclear trafficking of ER-a in MDECs on diethylstilbestrol treatment. DMSO-preexposed MDECs were exposed to 70 nmol/L diethylstilbestrol in the indicated time points. Translocation of ER-a protein (green) from the cytoplasm to the nucleus was observed, suggesting functional estrogen signaling. Nuclei were stained with 4¶,6-diamidino-2-phenylindole (DAPI; blue). The turquoise signals (merged) highlight the localization of ER-a in the nucleus.

Article Snippet: The MassARRAY platform was used to perform quantitative methylation analysis (Sequenom).

Techniques: Immunofluorescence, Solvent, Control, Expressing, Microarray, Chromatin Immunoprecipitation, Translocation Assay, Functional Assay, Staining